The objectives of the present studies was examined to elucidate the role of hepatic macrophages in liver repair after acute liver injury.
No associated publication
Specimen part
View SamplesInfliximab (IFX) has been reported as the further therapy in intravenous immunoglobulin G (IVIG)-resistant Kawasaki disease (KD) patients. IFX is a monoclonal antibody that blocks the pro-inflammatory cytokine tumor necrosis factor (TNF)-, but how IFX affect KD vasculitis is unknown. We investigated expression profiling of whole blood cells to elucidate the molecular mechanisms of the effectiveness of IFX therapy and to find characteristic biomarker and an important target in refractory KD. Methods: Refractory KD patients who failed to respond to repeated intravenous immunoglobulin G (IVIG) infusions had received a single infusion of IFX as third therapy. To validate specifically transcripts abundance for IFX therapy, we detected the altered transcripts expression and signaling pathways of whole blood mRNA in these IFX-responsive patients (n=8) using Affymetrix array, comparing initial IVIG-responsive patients (n=6).Results: A total of 1,388 transcripts abundance were significantly altered before and after IFX treatment. These transcripts abundance in IFX had Nucleotide-binding oligomerization domain pathway that play a role in activation of NFB and IL-1 signaling pathway outside the field of TNF- signaling pathway. Fifty transcripts including Peptidase inhibitor-3 (PI3), Matrix metalloproteinase-8 (MMP8), Chemokine (C-C motif) receptor-2 (CCR2) and Pentraxin-3 (PTX3) were significantly down-regulated in IFX. Conclusion: We demonstrated that the inhibition of TNF- by IFX have affected various molecular mechanism materially for IVIG-resistant KD patients.
Transcriptional regulation by infliximab therapy in Kawasaki disease patients with immunoglobulin resistance.
Specimen part, Disease, Disease stage, Treatment, Subject
View SamplesClinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone.
Clinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone.
Specimen part, Treatment, Subject, Time
View SamplesWe have tried to identify molecules associated with lymph node ratio expression by microarray. Expression microarray data prioritized gene candidates according to average expression ratio and its frequency.
Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer.
Sex, Age, Specimen part
View SamplesMetabolic syndrome is a collection of interconnected risk factors including obesity, insulin resistance, dyslipidemia and high blood pressure. Desirably, an effective pharmacotherapy for the medical condition should be able to target multiple risk factors concurrently. Based on our literature review and preliminary studies, ellagitannin geraniin, which is a polyphenolic compound, can confer many health benefits related to metabolic syndrome, but the underlying mechanism is unclear. Thus, we aim to explore the molecular pathways of the natural product using transcriptomic analysis. As for the experimental design, Sprague Dawley rats were given starch-based control diet (CD) or high-fat diet (HFD) for 8 weeks to induce metabolic syndrome. Then, some of the rats from HFD group were treated with ellagitannin gerannin (25 mg/kg/day) via oral gavage for 4 weeks. Other rats from the CD and HFD groups were treated with vehicle (10% w/v glucose solution) via the same approach. At the end of the experiment, total RNA was isolated from the liver for sequencing to compare the transcriptomes between groups.
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View SamplesEwg differentially regulated genes in 16-18 h Drosophila embryos. The experiment contains expression measurements from wild type, ewg l1 protein null allele and ewg l1 elavEWG (elavEWG rescue construct expressing a ewg cDNA from the elav promoter) mutants.
Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways.
Age
View Sampleswe aimed to explore the potential therapeutic effects of human mesenchymal stem cell on severe liver disease
No associated publication
Sex, Specimen part, Cell line
View SamplesAnalyze of RNA expression of Old Fibroblast and Young Fibroblast. Compare RNA expression of Old Fibroblast to RNA expression of Young Fbroblast
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Sex, Specimen part
View Sampleswe analysis of sham fibroblast and UVA fibroblast RNA expression using RNA sequencing and compare RNA expression.
No associated publication
Sex, Specimen part
View SamplesWe generate miR-25 KO mice by Cas-9 technology, and run 5 month kidney RNA sequencing.
No associated publication
Sex, Specimen part, Cell line
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