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Arabidopsis thaliana
28 Downloadable Samples
Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Effects of loss-of-function of AtMIKC* MADS-box genes on the mature Arabidopsis pollen transcriptome.
Publication Title
MADS-complexes regulate transcriptome dynamics during pollen maturation.
Sample Metadata Fields
Age, Specimen part
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SRP139292- Mus musculus
- 8 Downloadable Samples
Illumina HiSeq 2500
Description
Small RNA seq following over expression of miR-218 in HepG2 cells; mRNA expression following perturbation of miR-218 in primary mouse motor neurons
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
Progranulin mutations found in frontotemporal lobar degeneration (FTLD) cases typically result in heterozygous loss-of-function. To assess the impact of PGRN deficiency on gene expression, particularly of genes associated with lipid metabolism, in the brain, we sequenced the total RNA extracted from whole brains from 7-month-old female Grn+/+, Grn+/–, and Grn–/– mice and determined the differential expression of transcripts in PRGN-deficient (Grn+/- and Grn-/-) and wild type (Grn+/+) brains.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line
View Samples- Arabidopsis thaliana
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
Cuticular wax is a major composition of plant surface cuticle, which exerts crucial functions in optimizing plant growth. Histone acetylation regulates gene expression of diverse biological processes, but little is known about its role in cuticular wax synthesis. Here we observed that mutation of Arabidopsis histone acetyltransferase GCN5 gene impaired stem cuticular wax accumulation. Furthermore, three target genes of GCN5 (CER3, CER26 and CER1-L1) were identified by RNA-Seq and ChIP assays. Consistently, H3K9/14 acetylation levels in promoter regions of CER3, CER26 and CER1-L1 were significantly decreased in gcn5 as compared to wild type. Notably, overexpression of CER3 in the gcn5 mutant restored the stem cuticular wax accumulation. Collectively, these data demonstrate that Arabidopsis GCN5 is involved in stem cuticular wax accumulation by modulating CER3 expression via H3K9/14 acetylation, which present evidence that histone acetylation plays an important role in cuticular wax biosynthesis
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Arabidopsis thaliana
- 24 Downloadable Samples
- Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
Powdery mildew, caused by the fungus Blumeria graminis (DC) Speer, is one of the most important foliar diseases of cereals worldwide. It is an obligate biotrophic parasite, colonising leaf epidermal cells to obtain nutrients from the plant cells without killing them. Syringolin A (sylA), a circular peptide secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers a hypersensitive cell death reaction (HR) at infection sites when sprayed onto powdery mildew infected wheat which essentially eradicates the fungus. The rational was to identify genes whose expression was specifically regulated during HR, i.e. genes that might be involved in the switch of compatibility to incompatibility.<br></br>Powdery mildew-infected or uninfected plants were treated with syringolin two days after infection and plant material for RNA extraction was collected at 0.5, 1, 2, 4, 8, 12 hours after treatment (hat), resulting in an early (2 and 4 hat) and late pool (8 and 12 hat). Plant material that was uninfected prior to syringolin treatment was collected 8 and 12 hat (late pool of uninfected plant material), and 1 hat, respectively.
Publication Title
Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites.
Sample Metadata Fields
Compound, Time
View Samples- Danio rerio
- 12 Downloadable Samples
- IlluminaHiSeq2500
Description
RNA-seq-based profiling of Mycobacterium marinum granulomas from adult zebrafish and matched macrophage samples.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Hordeum vulgare
- 72 Downloadable Samples
- Affymetrix Barley Genome Array (barley1)
Description
The hypothesis whether CaCl2 seed treatment prior to sowing (osmopriming) can enhance drought stress tolerance, and/or alleviate the effect on plant growth was tested.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples SRP132125- Zea mays
- 7 Downloadable Samples
- Illumina HiSeq 2500
Description
To study the splicing factor role of ZmGRP1
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Disease stage, Cell line, Treatment
View Samples- Glycine max
- 9 Downloadable Samples
- Illumina HiSeq 2000
Description
To provide novel insights into the molecular basis of floral initiation, RNASeq was used to characterize the soybean transcriptome of leaf and micro-dissected shoot apical meristem at different time points after short-day treatment.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Saccharomyces cerevisiae
- 338 Downloadable Samples
- Illumina HiSeq 2500
Description
WT cells and mutants during growth on low phosphate levels and recovery
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Disease, Cell line
View Samples