This study is aimed to compare the gene expression between wild type and 35S:6MYC-HARP1 in response to wounding treatment.
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Age, Specimen part, Treatment
View SamplesTranscriptomic analysis of ZmUbi:ZmNAC111 transgenic maize under under well-watered and 2h dehydration stress conditions
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Specimen part
View SamplesTransposons in maize may be involved in the formation of circRNAs and further modulate phenotypic variation. To test our hypothesis, we performed circRNA-Seq(RNase R treated) on B73 seedlings(third leaves of V3 stage), and uncovered 1,572 high-confidence maize circRNAs, which show distinct genomic features compared to linear transcripts. Comprehensive analyses showed that LINE1-like elements (LLE) and their reverse complementary pairs (RCPLLEs) are significantly enriched in the flanking regions of circRNAs.
Circular RNAs mediated by transposons are associated with transcriptomic and phenotypic variation in maize.
Specimen part, Disease
View Samples1. To identify lncRNA regulating colorectal tumorigenesis, we performed RNA-seq analysis of cells with high and low tumorigenicity 2. To study the role of UPAT in colorectal cancer cells, we investigated the gene expression profiles of HCT116 cells in which UPAT expression had been suppressed by siRNA.
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Cell line
View SamplesIllumina/Solexa sequencing of CG3985 mutant and wildtype testes transcriptome (0-1 day males)
A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several Y-linked male fertility genes.
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View SamplesThe placenta has shown morphological and functional adaption in Meishan and Yorkshire pig breeds during late gestation. While the vast difference of uterine capacity and conceptus genotype between Western and Chinese pig breeds affects the pattern of placental development. Whether the placenta within Chinese pig breed also has similar adaptive changes is still unknown. So, we measured the weight and area of 80 Chinese indigenous Diannan small-ear pig placentas and further selected 6 placentas from extremely high and low litter size groups (HL and LL) for deep RNA-sequencing to detect the molecular basis relate to the observed placental phenotype difference.
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Sex, Specimen part
View SamplesBph6 is a gene that confers rice high resistance to its devastating pest BPH. Understanding the molecular responses of the resistant and susceptible varieties would pave the way to controlling the pest more effectively.
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Specimen part
View SamplesIn rice (Oryza sativa L.), the number of panicles, spikelets per panicle and grain weight are important components of grain yield. These characteristics are controlled by quantitative trait loci (QTLs) and are derived from variation inherent in crops.The identification of different yield related QTLs facilitates an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops.
Identification and characterization of OsEBS, a gene involved in enhanced plant biomass and spikelet number in rice.
Specimen part
View SamplesCD19 positive B cells were sorted from spleens in wild type mice and conditional konckout PRMT7 mice. RNA-seq experiments were performed to identify the differential expressing genes.
Histone Arginine Methylation by PRMT7 Controls Germinal Center Formation via Regulating Bcl6 Transcription.
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View SamplesAdipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. The recent explosion of knowledge have demonstrated that long noncoding RNA are involved in adipogenic gene regulatory network. However, existing annotations of lncRNAs involved in adipogenic differentiation are derived from preadipocyte cell lines, researches using primary cultures of farm animals are obviously required. To comprehensively identify lncRNAs with potential functions during bovine adipogenesis, in the present study we performed Ribo-Zero-Seq to survey the transcriptome landscape of in vitro cultured bovine preadipocytes and differentiated adipocytes. A stringent set of 2882 lncRNAs were finally identified. The 2882 lncRNAs shared many of the features of their mammalian counterparts: relatively shorter in length, significantly lower expression levels and fewer in exon number than RefSeq protein coding transcripts. Comparison of the lncRNAs expression profiles identified 16 specifically regulated lncRNAs during adipogenic differentiation. Integrative computational analyses associated these lncRNAs with several signaling pathways involved in lipid metabolism, including steroid biosynthesis, PPAR signaling pathway, glycolysis/gluconeogenesis, and fatty acid metabolism. Our data provide a valuable genomic resource for the identification of lncRNAs with potential functions in adipogenic differentiation.
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Sex, Specimen part
View Samples