To Identify new factors of GR-mediated mRNA decay.
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Sex, Age, Specimen part
View SamplesTIA1 and TIAL1 encode a family of U-rich-sequence-specific mRNA-binding proteins (mRBPs) ubiquitously expressed and conserved in metazoans. By PAR-CLIP, we determined that both proteins bind target sites with identical specificity in 3' UTRs as well as within introns proximal to 5' and 3' splice sites. Double knockout (DKO) of TIA1 and TIAL1 increased target mRNA abundance proportional to the number of binding sites and impacted the accumulation of aberrantly spliced mRNAs including the dsRNA-binding protein PRKRA, whose expression was completely blocked and subsequently triggered the activation of the dsRNA-activated protein kinase EIF2AK2/PKR and stress granule formation. Ectopic expression of PRKRA cDNA or knockout of EIF2AK2 in DKO cells rescued this phenotype. Perturbation of maturation and/or stability of additional targets also compromised cell cycle progression. Our study reveals the essential role of a single mRBP family contributing to fidelity of mRNA maturation, translation and RNA stress sensing pathways in human cells.
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View SamplesTranscriptome sequencing was performed for the chicken B-lymphoma DT40 cell line. rRNA-depletion of total RNA was done, a standard Illumina pair-end library was prepared and sequenced on Illumina HiSeq2000 and HiScan2000.
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Cell line
View SamplesThis project aims to delineate the circular RNA complement of mouse brain at age 8-9 weeks
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Sex, Age, Specimen part, Cell line
View SamplesNo description.
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Sex, Specimen part, Cell line, Treatment
View SamplesRNAseq of YAC128 mice treated with pridopidine
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Sex, Age, Specimen part, Cell line, Treatment
View SamplesFACS RNAseq of transgenic lines pWUS and pYAB
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Specimen part
View SamplesRaw sequence reads are provided for RNA-seq of parental and transgenerational worms in which the P0 were treated with OP50 (control) or PA14.
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Sex, Specimen part, Cell line
View SamplesIdentifying transcriptional changes in adults, whose biology and behavior differsubstantially from developing animals, is important when evaluating adult phenotypes.Moreover, cell- and tissue-specific information is critical for understanding the biologyof multicellular animals. We used adult cell-specific isolation to identify thetranscriptomes of C. elegans'' major adult tissues (muscle, intestine, epidermis, andneurons).
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Sex, Specimen part, Cell line
View SamplesWe evaluated the therapeutic activity of the modified U1 particles in a mouse model affected by severe spinal muscular atrophy. ExSpeU1 introduced by germline transgenesis efficiently rescued the phenotype increasing SMN2 exon 7 splicing, SMN protein production and radically extending the life span.
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Sex, Age, Specimen part, Disease, Cell line
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