submitter_institution:Universitat Marburg Results

Showing of 24 results

Sort by

Hide non-downloadable experiments

Filters

Technology

Microarray (645)

Platform

Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2) (342)

Affymetrix Porcine Genome Array (porcine) (143)

Affymetrix Yeast Genome 2.0 Array (yeast2) (36)

Affymetrix Human Exon 1.0 ST Array [transcript (gene) version, custom CDF (huex10st) (24)

Affymetrix Mouse Gene 1.0 ST Array (mogene10st) (24)

Affymetrix Human Genome U133A Array (hgu133a) (20)

Includes Publication (44)

E-MEXP-2885

Transcription profiling by array of yeast haploid Sigma1278b strains to detect Yak1-regulated genes

Saccharomyces cerevisiae
8 Downloadable Samples
Affymetrix Yeast Genome 2.0 Array (yeast2)

Description

We determined and classified Yak1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of different haploid MATa yeast strains of the following genotypes:YAK1 (468=YHUM0468), yak1 (1313=YHUM1313), strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 (1313Yhc) and strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 K398R (1313KDhc). All strains were grown in duplicate in YNB medium supplemented with tryptophan at 30 degrees Celsius to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.

Publication Title

No associated publication

Sample Metadata Fields

Sex

View Samples

E-MEXP-2810

Transcription profiling by array of yeast haploid Sigma1278b wildtype strain and whi3 deletion strain

Saccharomyces cerevisiae
4 Downloadable Samples
Affymetrix Yeast Genome 2.0 Array (yeast2)

Description

We determined and classified Whi3-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of two different haploid MATa yeast strains of the following genotypes: WHI3 strain (468=YHUM0468) and whi3 strain (1105=YHUM1105).Both starins were grown in duplicate in YNB medium with tryptophan at 30°C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

Publication Title

No associated publication

Sample Metadata Fields

Sex

View Samples

GSE69131

Gene Expression of primary rat hippocampal neurons after Ncoa3 knockdown

Rattus norvegicus
6 Downloadable Samples
Affymetrix Rat Gene 2.0 ST Array (ragene20st)

Description

We identified Ncoa3 as a regulator of neuronal morphology and microRNA activity. In order to uncover target genes of this transcriptional coactivator we performed this microarray analysis.

Publication Title

A large-scale functional screen identifies Nova1 and Ncoa3 as regulators of neuronal miRNA function.

Sample Metadata Fields

Specimen part, Treatment

View Samples

GSE62528

Site-specific methylation of Notch1 controls the amplitude and duration of the Notch1 response

Mus musculus
6 Downloadable Samples
Affymetrix Mouse Gene 1.0 ST Array (mogene10st)

Description

Physiologically, Notch signal transduction plays a pivotal role in differentiation; pathologically, Notch signaling contributes to the development of cancer. Transcriptional activation of Notch target genes involves cleavage of the Notch receptor in response to ligand binding, production of the Notch intracellular domain (NICD), and NICD migration into the nucleus and assembly of a coactivator complex. Posttranslational modifications of the NICD are important for its transcriptional activity and protein turnover. Deregulation of Notch signaling and stabilizing mutations of Notch1 have been linked to leukemia development. We found that the methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1; also known as PRMT4) methylated NICD at five conserved arginine residues within the C-terminal transactivation domain. CARM1 physically and functionally interacted with the NICD-coactivator complex and was found at gene enhancers in a Notch-dependent manner. Although a methylation-defective NICD mutant was biochemically more stable, this mutant was biologically less active as measured with Notch assays in embryos of Xenopus laevis and Danio rerio. Mathematical modeling indicated that full but short and transient Notch signaling required methylation of NICD.

Publication Title

Site-specific methylation of Notch1 controls the amplitude and duration of the Notch1 response.

Sample Metadata Fields

Cell line

View Samples

E-MEXP-3610

Transcription profiling by array of Saccharomyces cerevisiae (yeast) to analyse and classify Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions

Saccharomyces cerevisiae
6 Downloadable Samples
Affymetrix Yeast Genome 2.0 Array (yeast2)

Description

We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling

Publication Title

No associated publication

Sample Metadata Fields

Sex, Subject

View Samples

GSE14739

Impact of breed and sex on porcine endocrine transcriptome: A Bayesian biometrical analysis

Sus scrofa
80 Downloadable Samples
Affymetrix Porcine Genome Array (porcine)

Description

Background: Transcriptome variability is due to genetic and environmental causes, much like any other complex phenotype. Ascertaining the transcriptome differences between individuals is an important step to understand how selection and genetic drift may affect gene expression. To that end, extant divergent livestock breeds offer an ideal genetic material.

Publication Title

Impact of breed and sex on porcine endocrine transcriptome: a bayesian biometrical analysis.

Sample Metadata Fields

Sex, Specimen part

View Samples

GSE10898

Transcriptome architecture across tissues in the pig

Sus scrofa
63 Downloadable Samples
Affymetrix Porcine Genome Array (porcine)

Description

Artificial selection has resulted in animal breeds with extreme phenotypes. As an organism is made up of many different tissues and organs, each with its own genetic programme, it is pertinent to ask what are the relative contributions of breed or sex when assessed across tissues.