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Mus musculus
8 Downloadable Samples
Affymetrix Murine Genome U74A Version 2 Array (mgu74av2)
Description
To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays.
Publication Title
Expression profiling of cumulus cells reveals functional changes during ovulation and central roles of prostaglandin EP2 receptor in cAMP signaling.
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 6 Downloadable Samples
- Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2)
Description
Rationale Electroconvulsive seizure (ECS) therapy is a nonchemical treatment for depression. Since ECS up-regulates expression of c-Fos in the paraventricular nucleus of hypothalamus (PVN), the function of which is frequently influenced in depression, we hypothesized that ECS modulates functions of the PVN and contributes to its antidepressant effects. Objectives To identify gene expression changes in the mouse PVN by ECS treatment Material and methods First, we established a method to amplify nucleotides from small quantities of RNA. Mice received one shock of ECS and their brains were collected at 2 or 6 h after shock. The PVN was microdissected from dehydrated brain sections, its total RNA was extracted and microarray analysis was applied. Results At 2 h after ECS, 2.6% (589 genes) of the probes showed more than 2-fold decrease, and 0.9% (205 genes) showed more than 2-fold increase. To confirm the expression changes, genes showing differential expression with a wide range in the microarray were analyzed by qPCR. Among the genes with more than 2-fold change by ECS, down-regulated 94 genes and up-regulated 24 genes have been reported the association with anxiety, bipolar disorder or mood disorder by the Ingenuity knowledge database. The groups of down-regulated genes, which are suggested to modulate the function of the PVN or associate to psychiatric disorders, include neuropeptides (Cck), kinases (Prkcb, Prkcc, Camk2a), transcription factors (Bcl6, Tbr1), transporters (Aqp4) and others (Fmr1). Conclusion The present results indicate that ECS treatment can modulate the functions of PVN via a series of gene expression changes, and may contribute to its antidepressant effects at least in part.
Publication Title
Electroconvulsive seizure-induced changes in gene expression in the mouse hypothalamic paraventricular nucleus.
Sample Metadata Fields
Specimen part, Treatment, Time
View Samples- Mus musculus
- 5 Downloadable Samples
- Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2)
Description
The ventromedial nucleus of the hypothalamus (VMH) is thought to a satiety center and a potential target for anti-obesity therapy. Electroconvulsive seizure (ECS) therapy is highly effective in psychiatric diseases including depression, but also implicated beneficial effects on other neurological diseases. Although it has been reported that the neurons in the VMH are strongly activated by ECS stimulation, the effect of ECS in this hypothalamic subnucleus remains unknown. To address this issue, we investigated molecular changes in the VMH in response to ECS by utilizing a method of laser-capture microdissection coupled with microarray analysis, and examined behavioral effects of ECS via VMH activation. ECS significantly induced gene expression not only immediate-early genes such as Fos, Fosb and Jun, but also Bdnf, Adcyap1, and Hrh1 in the VMH after a single or repeated stimulus.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 4 Downloadable Samples
- Affymetrix Mouse Gene 1.0 ST Array (mogene10st)
Description
Transcriptome analysis of LPS-stimulated bone marrow-derived dendritic cells with NR4A3 gene silencing
Publication Title
The Orphan Nuclear Receptor NR4A3 Is Involved in the Function of Dendritic Cells.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 4 Downloadable Samples
- Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2)
Description
The dentate gyrus (DG) of the hippocampus is one of major targets for antidepressant treatments. Using electroconvulsive stimulation (ECS), a model of highly effective and fast-acting antidepressant therapy, here we show that neural stimulation via ECS induces rapid and lasting dematuration of granule neurons in DG. A single time of stimulation transiently reduced mature marker expression and mature synaptic functions. Repetitive stimulation converted this transient dematuration into a stable form lasting more than 1 month. We compared the activity-dependent neuronal responsiveness in the DG between a single ECS and repeated ECS.
Publication Title
Rapid and stable changes in maturation-related phenotypes of the adult hippocampal neurons by electroconvulsive treatment.
Sample Metadata Fields
Specimen part, Disease, Treatment
View Samples- Mus musculus
- 4 Downloadable Samples
- Affymetrix Mouse Genome 430A 2.0 Array (mouse430a2)
Description
The dentate gyrus (DG) of the hippocampus is one of major targets for antidepressant treatments. Our recent research has revealed that selective serotonin reuptake inhibitor (SSRI) treatment causes a long-lasting change in the phenotypes of mature dentate granule neurons to immature state in adult mouse DG. However, it is unknown whether this dematuration of DG is a common effect of antidepressant treatments and what mechanisms underlie it. Using electroconvulsive stimulation (ECS), a model of highly effective and fast-acting antidepressant therapy, here we show that neural stimulation via ECS induces rapid and lasting dematuration of granule neurons in DG. A single or few times of stimulation transiently reduced mature marker expression and mature synaptic functions. Repetitive stimulation converted this transient dematuration into a stable form lasting more than 1 month. Dematured granule neurons showed higher excitability, and an increase in GABA-mediated inhibition by the benzodiazepine diazepam prevented the lasting maintenance phase of dematuration without affecting the initial induction phase. Our study suggests that dematuration of DG is a common cellular mechanism underlying effects of different types of antidepressant treatments, and demonstrate a novel role for excitation/inhibition balance in bidirectional regulation of the state of neuronal maturation in the adult brain.
Publication Title
Rapid and stable changes in maturation-related phenotypes of the adult hippocampal neurons by electroconvulsive treatment.
Sample Metadata Fields
Specimen part
View Samples- Rattus norvegicus
- 15 Downloadable Samples
Illumina HiSeq 4000
Description
Metabolic syndrome is a collection of interconnected risk factors including obesity, insulin resistance, dyslipidemia and high blood pressure. Desirably, an effective pharmacotherapy for the medical condition should be able to target multiple risk factors concurrently. Based on our literature review and preliminary studies, ellagitannin geraniin, which is a polyphenolic compound, can confer many health benefits related to metabolic syndrome, but the underlying mechanism is unclear. Thus, we aim to explore the molecular pathways of the natural product using transcriptomic analysis. As for the experimental design, Sprague Dawley rats were given starch-based control diet (CD) or high-fat diet (HFD) for 8 weeks to induce metabolic syndrome. Then, some of the rats from HFD group were treated with ellagitannin gerannin (25 mg/kg/day) via oral gavage for 4 weeks. Other rats from the CD and HFD groups were treated with vehicle (10% w/v glucose solution) via the same approach. At the end of the experiment, total RNA was isolated from the liver for sequencing to compare the transcriptomes between groups.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples
SRP136224- Danio rerio
- 12 Downloadable Samples
- IlluminaHiSeq2500
Description
To reveal the toxic mechanism of thifluzamide in zebrafish
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Pseudomonas aeruginosa
- 2 Downloadable Samples
- NextSeq 500
Description
We undertook to validate the therapeutic use of a polyherbal preparation described in Ayurved as ‘Panchvalkal’, which has been suggested for treatment of microbial infections. We tested this formulation available in market as Pentaphyte P-5®, against Pseudomonas aeruginosa, for its quorum sensing (QS) modulatory potential. Following demonstration of its in vitro QS modulatory potential, we assayed it for in vivo efficacy using the nematode Caenorhabditis elegans as a model host. This polyherbal formulation conferred notable survival benefit when the nematode worm was challenged with the test pathogen. Todecipher the molecular basis of its efficacy, whole transcriptome analysis of P. aeruginosa exposed to ‘Panchvalkal’ was done, its gene expression profile inpresence of ‘Panchvalkal’ was compared with that in its absence. This polyherbal formulation also enhanced the susceptibility of P. aeruginosa to antibiotics like gentamicin, tetracycline, and cephalexin. This study is a good demonstration of the role of bacterial QS machinery as an important target for development of new antimicrobials/ anti-infectives, and also of the effective use of moderngenomics for validation of ayurvedic prescriptions i.e. ayuromics.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Disease
View Samples SRP115073- Danio rerio
- 1 Downloadable Sample
- Illumina HiSeq 2500
Description
The study was to unravel the complex toxic effects of chemical contaminants on the health of organisms as based on different levels of biological profling.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples