Human primordial germ cells and mouse neonatal and adult germline stem cells are pluripotent and derive embryonic stem cell properties.
Generation of pluripotent stem cells from adult human testis.
No sample metadata fields
View SamplesThis is the first study to investigate mRNA expression profiling in regard to hepatic I/R and IPO by next-generation RNA-Seq. Our results may provide an experimental basis for elucidating the underlying mechanism of IPO and reveal candidate biomarkers with which to assess hepatic I/R injury Overall design: liver mRNA profiles of sham, I/R and IPO mice were generated by next-generation sequencing, in triplicate, using Illumina HiSeq 4000.
Gene Expression Profiling in Ischemic Postconditioning to Alleviate Mouse Liver Ischemia/Reperfusion Injury.
Specimen part, Cell line, Treatment, Subject
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Thymic negative selection is functional in NOD mice.
Sex, Age
View SamplesThe aim of this study was to quantify the impact of NOD genetic vatiation on thymic negative selection transcriptional programs.
Thymic negative selection is functional in NOD mice.
Sex, Age
View SamplesThe aim of this study was to quantify the impact of NOD genetic vatiation on the transcriptional programs induced by the alpha beta-TCR at the DN to DP transition in the BDC2.5 TCR Tg model
Thymic negative selection is functional in NOD mice.
Sex, Age
View SamplesPurpose: To compare the transcriptome of MESP1-mTomato reporter cells at undifferentiated state, cardiac differentiation day 3 and day 5. Methods: total RNA from sorted MESP1+, MESP1- and HESCs (in biological duplicates) was extracted using RNeasy Plus Mini Kit (Qiagen) and treated with RNase free DNase. RNA library was prepared following the instruction of TruSeqâ„¢ RNA Sample Preparation kit (Illumina) and sequenced on Illumina HiSeq 2000. Results: genes differentially expressed in MESP1-mTomato+ and mTomato- cells were identified. Conclusions: the gene expression profile of MESP1-mTomato cells indicates that they are cardiovascular progenitor cells. Overall design: Through RNA-seq of MESP1+, MESP1- and undifferentiated cells, perform bioinformatics analysis to identify differentially expressed genes, then perform functional study.
Homozygous MESP1 knock-in reporter hESCs facilitated cardiovascular cell differentiation and myocardial infarction repair.
No sample metadata fields
View SamplesObjective: To study the physiological role of eosinophils in the GI tract and lung under homeostatic conditions,
The pan-B cell marker CD22 is expressed on gastrointestinal eosinophils and negatively regulates tissue eosinophilia.
Specimen part
View SamplesThe eosinophil transcriptome analysis indicated a robust transcription change in eosinophils following allergen challenge in the lung.
Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
Specimen part
View SamplesMorphogenesis of epithelial tissues relies on the precise developmental control of cell polarity and architecture. In the early Drosophila embryo, the primary epithelium forms during cellularisation, following a tightly controlled genetic programme where specific sets of genes are up-regulated. Some of them, for instance, control membrane invagination between the nuclei anchored at the apical surface of the syncytium.
IL-13 induces esophageal remodeling and gene expression by an eosinophil-independent, IL-13R alpha 2-inhibited pathway.
Specimen part
View SamplesWe generated RNA profiling datasets from adult mouse dentate gyrus with cFos knockdown and/or overexpression upon neuronal activation Overall design: Examination of chromatin accessibility changes and RNA expression changes in mouse dentate gyrus upon neuronal activation
Neuronal activity modifies the chromatin accessibility landscape in the adult brain.
Specimen part, Cell line, Subject
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