Global transcriptome patterns were performed using SHYG-IOE-5h (5h after Estradiol and Mock treatment)
NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis.
Specimen part, Treatment
View SamplesGlobal transcriptome patterns were performed using ORE1-IOE-2h (2h after Estradiol and Mock treatment) as well as transiently (6h) overexpressed Arabidopsis mesophyll cell protoplasts
NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis.
Specimen part, Treatment
View SamplesElucidating the top of the mammary epithelial cell hierarchy is highly important for understanding its regeneration capabilities and identifying target cells for transformation. Aiming for enriched mammary epithelial stem cell population, CD200highCD200R1high epithelial cells were identified. These cells represent ~50% of the mammary repopulating units (MRUs, CD49fhigh CD24med ) and termed MRUCD200/CD200R1. Gene expression of these cells was compared to all other MRU cells, termed MRUnot CD200/CD200R1, as well as individual CD200+ population (MRU-CD200R1-) and CD200R1+ population (MRU-CD200-). Overall design: Gene expression from mammary epithelial cells carrying sorted by CD200, CD200R1 markers and MRU markers. Four populations were sequenced: MRU-positive CD200 positive and CD200R1 positive; MRU-positive and not CD200 positive CD200R1 positive; not MRU CD200 positive CD200R1 negative; not MRU CD200 negative CD200R1 positive. There are 5 replicates from 5 individual mice.
High Expression of CD200 and CD200R1 Distinguishes Stem and Progenitor Cell Populations within Mammary Repopulating Units.
Sex, Specimen part, Cell line, Subject
View SamplesA transgenic mouse was generated using a CD2-driven transgene containing the cDNA of Ppp2ca to achieve over-expression of PP2Ac in T cells. Nave CD4 T cells were isolated and lysed at times 0, 6, and 24 hours after stimulation with anti-CD3 and anti-CD28
Protein phosphatase 2A enables expression of interleukin 17 (IL-17) through chromatin remodeling.
No sample metadata fields
View SamplesRNA expression was measured by RNA-seq in E17 wild type and Sall1-?SRM mutant kidney. Overall design: RNA expression in mutant kidney was compared to wild type stage matched kidney.
A Sall1-NuRD interaction regulates multipotent nephron progenitors and is required for loop of Henle formation.
Specimen part, Subject
View SamplesCF patients suffer from chronic and recurrent respiratory tract infections which eventually lead to lung failure followed by death. Pseudomonas aeruginosa is one of the major pathogens for CF patients and is the principal cause of mortality and morbidity in CF patients.
Bacterial adaptation during chronic infection revealed by independent component analysis of transcriptomic data.
No sample metadata fields
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.
Specimen part
View SamplesInfluenza virus infection-induced gene expression changes of regional B cells are mediated at least in part through type I Interferon:
Influenza virus infection causes global respiratory tract B cell response modulation via innate immune signals.
Sex, Specimen part
View SamplesTranscriptional profiling of the zebrafish embryonic host response to a systemic bacterial infection with Salmonella typhimurium (strain SL1027); comparison between traf6 knock-down and control morpholino treated embryos. Overall design: All infection experiments were performed using mixed egg clutches of ABxTL strain zebrafish. Embryos injected with traf6 morpholino or a 5bp mismatch control morpholino were staged at 27 hours post fertilization (hpf) by morphological criteria and approximately 250 cfu of DsRed expressing Salmonella bacteria were injected into the caudal vein close to the urogenital opening. As a control an equal volume of PBS was likewise injected. Pools of 20-40 infected and control embryos were collected 8 hours post infection (hpi). The whole procedure was preformed in triplicate on separate days. Total RNA of the biological triplicates was pooled using equal amounts of RNA prior to RNAseq library preparation.
Transcriptome analysis of Traf6 function in the innate immune response of zebrafish embryos.
No sample metadata fields
View SamplesImprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally expressed alleles are distinguished epigenetically by DNA methylation and histone modifications.
Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.
Specimen part
View Samples