This study characterizes the inflammatory processes in left-sided colitis, pancolitis, and UC-associated dysplasia at the transcriptional level in colonics biopsies in order to identify potential biomarkers and transcripts of importance for the carcinogenic behaviour of chronic inflammation
Transcriptional analysis of left-sided colitis, pancolitis, and ulcerative colitis-associated dysplasia.
Specimen part, Disease, Disease stage
View SamplesThe epithelial expression of the insulin receptor in the colon is previously reported to correlate with the extent of colonic inflammation. Here, we investigated the effect of inactivating the epithelial insulin receptor in the intestinal tract, in an experimental model of inflammation-induced colorectal cancer. We report increased susceptibility to chemically-induced colitis together with potentiated colonic tumorigenesis in the knockout mice. Furthermore, we show that topically administered insulin in inflamed colons of wildtype mice reduces inflammation-induced weight loss and improves remission in a dose-dependent manner. Mice receiving rectal insulin enemas exhibited lower colitis endoscopic scores and developed significantly fewer and smaller tumors compared with the control group receiving phosphate-buffered saline only. Rectal insulin therapy can potentially be a novel treatment targeting the epithelial layer to enhance mucosal healing in the ulcerated areas. Our findings open up new possibilities for combination treatments to synergize with the existing anti-inflammatory therapies.
Rectal Insulin Instillation Inhibits Inflammation and Tumor Development in Chemically Induced Colitis.
Treatment
View SamplesTumor-associated blood vessels differ from normal vessels at the morphological and molecular level. Proteins that are only present on tumor vessels may serve as biomarkers and as therapeutic targets for inhibition of angiogenesis in cancer. Comparing the transcriptional profiles of blood vascular endothelium from human invasive bladder cancer and from normal bladder tissue, we found several markers that could serve as novel biomarkers or therapeutic targets.
Endocan is upregulated on tumor vessels in invasive bladder cancer where it mediates VEGF-A-induced angiogenesis.
Sex, Disease stage
View SamplesOGR1 is a pH-sensing G-protein coupled receptor involved in intestinal homeostasis and inflammation
The pH-sensing receptor OGR1 improves barrier function of epithelial cells and inhibits migration in an acidic environment.
Specimen part, Cell line, Treatment
View SamplesOGR1 is a pH sensing G protein-coupled receptor involved in intestinal homeostasis and inflammation. Up-regulation of genes, mediated by OGR1, in response to extracellular acidification were enriched for inflammation, immune response, actin cytoskeleton and cell adhesion pathways.
G Protein-coupled pH-sensing Receptor OGR1 Is a Regulator of Intestinal Inflammation.
Specimen part, Treatment
View SamplesThe goal of our study is to determine whether Atg16L1 deficiency leads to differences in the transcriptional profile of CD11c+ Dendritic Cells, ultimately leading to an increased inflammatory phenotype.
Autophagy gene Atg16L1 prevents lethal T cell alloreactivity mediated by dendritic cells.
Specimen part
View SamplesThe goal of our study is to determine whether Atg16L1 deficiency leads to differences in the transcriptional profile of CD11c+ Dendritic Cells, ultimately leading to an increased inflammatory phenotype.
Autophagy gene Atg16L1 prevents lethal T cell alloreactivity mediated by dendritic cells.
Age, Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Autophagy gene Atg16L1 prevents lethal T cell alloreactivity mediated by dendritic cells.
Age, Specimen part
View SamplesLncRNA H19X was silienced in dermal fibroblats of systemic sclerosis patients with antisense oligonuclotides. The hypothesis tested in the present study was that H19X is an important factor in the development of TGFb-driven fibrosis. Results provide important information about the role H19X in fibroblasts in particolar on extracellular matrix production and cell cycle regulation.
Long noncoding RNA H19X is a key mediator of TGF-β-driven fibrosis.
Specimen part, Disease, Disease stage, Treatment
View SamplesAging has been shown to be under genetic control in C. elegans. We performed Affymetrix micorarray-based transcriptional profililng of wild type C. elegans strain Bristol N2 during aging to detect temporal changes in gene expression.
A decline in p38 MAPK signaling underlies immunosenescence in Caenorhabditis elegans.
Specimen part
View Samples