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GSE63372
Arabidopsis thaliana
18 Downloadable Samples
Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)
Description
In order to study the improtance of HSFA6b in salt and heat stresses of arabidopsis, we generated the HSFA6b-overexpression (HSFA6b-OE) and dominant-negative (HSFA6b-RD) mutant lines.
Publication Title
The Heat Stress Factor HSFA6b Connects ABA Signaling and ABA-Mediated Heat Responses.
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 9 Downloadable Samples
Illumina HiSeq 4000
Description
This is the first study to investigate mRNA expression profiling in regard to hepatic I/R and IPO by next-generation RNA-Seq. Our results may provide an experimental basis for elucidating the underlying mechanism of IPO and reveal candidate biomarkers with which to assess hepatic I/R injury Overall design: liver mRNA profiles of sham, I/R and IPO mice were generated by next-generation sequencing, in triplicate, using Illumina HiSeq 4000.
Publication Title
Gene Expression Profiling in Ischemic Postconditioning to Alleviate Mouse Liver Ischemia/Reperfusion Injury.
Sample Metadata Fields
Specimen part, Cell line, Treatment, Subject
View Samples- Homo sapiens
- 8 Downloadable Samples
- Affymetrix Human Exon 1.0 ST Array [transcript (gene) version (huex10st)
Description
The tumor suppressor protein p53 functions to regulate diverse cellular processes including apoptosis, cell cycle, senescence and metabolism. Act as a master transcription factor, it can transcriptionally regulate the gene expression of many downstream target genes. p53 is frequently mutated in cancer by single nucleotide/amino acid substitutions at the DNA binding domain that result in a mutant form of p53 protein and usually loses its DNA binding and transcriptional regulation abilities.
Publication Title
p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1.
Sample Metadata Fields
Specimen part, Cell line
View Samples- Mus musculus
- 14 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
Homeostatic hematopoietice stem cells (HSCs) with greater divisional history lose repopulating potential after very few cell divisions. Divisional history overrides both phenotype and immediate quiescence in determining functional activity. In GFP label retaining system GFP is progressively diluted when cells proceed through a cascade of divisions.
Publication Title
Divisional history and hematopoietic stem cell function during homeostasis.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 12 Downloadable Samples
- Illumina HiSeq 2500
Description
A comparison of the mRNAs analysis in lactation mammary myoepithelial cells (GFP+/GFP-) and brown adipocytes (GFP+) from Myf5-Cre-ROSAmTmG and Ucp1-iCre-ROSAmTmG mice. Results provide the gene expression signature in the brown origin (UCP1/Myf5-positive) myoepithelial cells in vivo. Overall design: Mammary myoepithelial cells and brown adipocytes were isolated by FACS sorting based on the cell surface markers and total RNA were immediately. GFP+ mammary myoepithelial cells (Lin-:CD24+:CD29hi) from lactation day 10 Myf5-Cre-ROSAmTmG mice (n=2). GFP- mammary myoepithelial cells (Lin-:CD24+:CD29hi) from lactation day 10 Myf5-Cre-ROSAmTmG mice (n=2). GFP+ mammary myoepithelial cells (Lin-:CD24+:CD29hi) from lactation day 10 Ucp1-iCre-ROSAmTmG mice (n=2). GFP- mammary myoepithelial cells (Lin-:CD24+:CD29hi) from lactation day 10 Ucp1-iCre-ROSAmTmG mice (n=2). GFP+ brown adipocytes from 8-week old female Myf5-Cre-ROSAmTmG mice (n=2). GFP+ brown adipocytes from 8-week old female Ucp1-iCre-ROSAmTmG mice (n=2).
Publication Title
Brown adipocytes can display a mammary basal myoepithelial cell phenotype in vivo.
Sample Metadata Fields
Specimen part, Cell line, Subject
View Samples- Homo sapiens
- 5 Downloadable Samples
- Affymetrix Human Gene 2.0 ST Array (hugene20st)
Description
Benzene is a ubiquitous environmental pollutant and an established human hematotoxicant and leukemogen. New insights into the pathogenesis of benzene hematotoxicity are urgently needed. Long non-coding RNAlncRNAcan regulate gene expression and widely participate in the various physiological and pathological processes.
Publication Title
Long non-coding RNA NR_045623 and NR_028291 involved in benzene hematotoxicity in occupationally benzene-exposed workers.
Sample Metadata Fields
Specimen part, Treatment
View Samples- Arabidopsis thaliana
- 8 Downloadable Samples
- Illumina Genome Analyzer IIx
Description
To investigate possible smRNAs linked to TMM silencing by single-stranded and double-stranded silencers, we determined sequences of smRNAs by the Illumina high-throughput sequencing platform and compared silencing efficiency of different strategies. We found that single-stranded silencer alone could promote the production of smRNAs. Overall design: smRNA profiles of 2-week-old wide type seedlings (WT) and different silencers were generated by Illumina Genome Analyzer IIx.
Publication Title
Transcriptional silencing of Arabidopsis endogenes by single-stranded RNAs targeting the promoter region.
Sample Metadata Fields
Specimen part, Subject
View Samples- Homo sapiens
- 6 Downloadable Samples
- Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
Ameloblast differentiation is the most critical stepwise process in amelogenesis, and it is controlled by precise molecular events. To better understand the mechanism controlling pre-ameloblasts (PABs) differentiation into secretory ameloblasts (SABs), a more precise identification of molecules and signaling networks will elucidate the mechanisms governing enamel formation and lay a foundation for enamel regeneration.
Publication Title
Cell cycle control, DNA damage repair, and apoptosis-related pathways control pre-ameloblasts differentiation during tooth development.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina Genome Analyzer
Description
We have previously reported that radix Angelica sinensis (RAS) suppressed body weight and altered FTO expression in mice with high fat diet (HFD)-induced obesity. In the present study we performed RNA sequencing-mediated transcriptome analysis to elucidate the molecular mechanisms underlying the anti-obesogenic effects of RAS in mice. The results revealed that 36 differentially-expressed genes (DEGs) were identified between the RAS supplementation group (DH) and control group (HC). These 36 DEGs were clustered into 297 functional gene ontology (GO) categories, among which several GO annotations and signaling pathways were associated with lipid homeostasis. Six out of the 36 DEGs were identified to be involved in lipid metabolism, with the APOA2 gene a potential anti-obesogenic influence. The expression pattern revealed by RNA-Seq was identical to the results of quantitative real-time PCR (qPCR). Therefore, RAS supplementation in HFD-induced obese mice was associated with an anti-obesogenic global transcriptomic response. This study provides insight into potential applications of RAS in obesity therapy. Overall design: Examination ofdifferentially-expressed genes in 2 groups.
Publication Title
Anti-obesity effect of radix Angelica sinensis and candidate causative genes in transcriptome analyses of adipose tissues in high-fat diet-induced mice.
Sample Metadata Fields
Cell line, Subject
View Samples- Mus musculus
- 5 Downloadable Samples
- Illumina Genome Analyzer II
Description
MicroRNAs (miRs) play a key role in the control of gene expression in a wide array of tissue systems where their functions include the regulation of self-renewal, cellular differentiation, proliferation, and apoptosis. However, the functional importance of individual miRs in controlling spermatogonial stem cell (SSC) homeostasis has not been investigated. Using high-throughout sequencing, we profiled the expression of miRs in the Thy1+ testis cell population, which is highly enriched for SSCs, and the Thy1- cell population, composed primarily of testis somatic cells. In addition, we profiled the global expression of miRs in cultured germ cells, also enriched for SSCs. Our results demonstrate that miR-21, along with miR-34c, -182, -183, -146a, -465a-3p, -465b-3p, -465c-3p, and -465c-5p are preferentially expressed in the Thy1+ SSC-enriched population, as compared to Thy1- somatic cells, and we further observed that Thy1+ SSC-enriched testis cells and SSC-enriched cultured germ cells share remarkably similar miR expression profiles. Overall design: Spermatogonial Stem Cell enriched cell populations (freshly isolated and short-term cultured) and somatic cell populations were isolated from C57B/L6 mouse donors and subjected to small RNA isolation and sequencing.
Publication Title
MicroRNA-21 regulates the self-renewal of mouse spermatogonial stem cells.
Sample Metadata Fields
Specimen part, Cell line, Subject
View Samples